Novak F J, Gräf R, Waring R B, Wolfersberger M G, Wieczorek H, Harvey W R
Department of Biology, Temple University, Philadelphia, PA 19122.
Biochim Biophys Acta. 1992 Aug 17;1132(1):67-71. doi: 10.1016/0167-4781(92)90053-3.
The amino acid sequence of a vacuolar-type ATPase (V-ATPase) subunit B has been deduced from a cDNA clone isolated from a Manduca sexta larval midgut library. The library was screened by hybridization with a labeled cDNA encoding subunit B of Arabidopsis thaliana tonoplast V-ATPase. The M. sexta V-ATPase subunit B consists of 494 amino acids with a calculated M(r) of 54,902. The amino acid sequence deduced for V-ATPase subunit B of M. sexta is between 98% and 76% identical with that of seven other V-ATPase subunits B and greater than 52% identical with three archaebacterial ATPase subunits B.
