Gräf R, Novak F J, Harvey W R, Wieczorek H
Zoologisches Institut der Universität, München, Germany.
FEBS Lett. 1992 Mar 30;300(2):119-22. doi: 10.1016/0014-5793(92)80177-i.
For the first time a cDNA encoding subunit A of an invertebrate V-ATPase has been sequenced. The cDNA library was prepared from larval midgut of the tobacco hornworm, Manduca sexta, and screened with monoclonal antibodies to the midgut plasma membrane subunit A. From the cDNA sequence the insect subunit A is predicted to consist of 617 amino acids with a relative molecular mass of 68,162. The predicted primary structure is similar to that of the published eukaryotic subunit A proteins (Bos, Daucus, Saccharomyces and Neurospora); it most closely resembles the bovine amino acid sequences with which it has an 83% sequence identity.
首次对编码无脊椎动物V - ATP酶A亚基的cDNA进行了测序。cDNA文库是从烟草天蛾（Manduca sexta）幼虫中肠制备的，并用针对中肠质膜A亚基的单克隆抗体进行筛选。根据cDNA序列预测，昆虫A亚基由617个氨基酸组成，相对分子质量为68,162。预测的一级结构与已发表的真核生物A亚基蛋白（Bos、Daucus、Saccharomyces和Neurospora）相似；它与牛的氨基酸序列最为相似，序列同一性为83%。
