Morikawa K, Oseko F, Morikawa S
Department of Internal Medicine, Shimane Medical University, Japan.
Int J Immunopharmacol. 1992 Jul;14(5):941-9. doi: 10.1016/0192-0561(92)90094-2.
A biscoclaurine alkaloid, cepharanthine, is known to be a potent inhibitor of snake venom-induced hemolysis by interaction with the lipid bilayer of the membrane. The drug also interferes with the ion channel intracellularly. In this study, we examined the effect of cepharanthine on human B-cell functions. Small dense B-cells from tonsil samples were isolated using a Percoll density gradient from non-rosetted cells and were used as the target cells. Cepharanthine inhibited the proliferation of the lymphocytes and antibody production of human B-lymphocytes. The inhibitory effect of cepharanthine on the proliferation was caused by the arrest of the late G1 to S phase transition in the cell cycle. However, the mechanism of suppression of antibody formation remains unknown. These results suggest that cepharanthine acts on human B-cells as an immunomodulating agent.
双苄基异喹啉生物碱汉防己甲素是一种已知的通过与膜脂质双层相互作用来有效抑制蛇毒诱导的溶血的物质。该药物还在细胞内干扰离子通道。在本研究中，我们检测了汉防己甲素对人B细胞功能的影响。使用Percoll密度梯度从非玫瑰花结形成细胞中分离扁桃体样本中的小而致密的B细胞，并将其用作靶细胞。汉防己甲素抑制淋巴细胞增殖和人B淋巴细胞的抗体产生。汉防己甲素对增殖的抑制作用是由细胞周期中G1晚期至S期转换的停滞引起的。然而，抗体形成的抑制机制仍不清楚。这些结果表明，汉防己甲素作为一种免疫调节剂作用于人B细胞。
