尿素对烟草花叶病毒的作用。II. 蛋白质亚基之间的键
Action of urea on tobacco mosaic virus. II. The bonds between protein subunits.
作者信息
BUZZELL A
出版信息
Biophys J. 1962 Mar;2(2 Pt 1):223-33. doi: 10.1016/s0006-3495(62)86851-9.
Abstract
The rate of urea degradation is not uniform over the tobacco mosaic virus (TMV) rod, as might be expected since the protein subunits appear to be chemically identical. Only two stable intermediates are formed; the four components with sedimentation constants, S(40) (20), about 80, 120, 140, and 165 correspond to the two intermediates with and without RNA tails. Analysis of the reaction kinetics for the disappearance of whole virus and of the first intermediate was made in terms of a new hypothesis concerning protein bonding.
摘要
正如可能预期的那样，由于蛋白质亚基在化学上似乎是相同的，尿素在烟草花叶病毒（TMV）棒上的降解速率并不均匀。只形成了两种稳定的中间体；沉降常数S(40) (20)分别约为80、120、140和165的四个组分对应于带有和不带有RNA尾巴的两种中间体。根据关于蛋白质结合的新假说，对完整病毒和第一种中间体消失的反应动力学进行了分析。
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