Jiang H, Takahara S, Kyo M, Takano Y, Kokado Y, Ishibashi M, Okuyama A, Sonoda T
Department of Urology, Osaka University Medical School, Japan.
Transpl Int. 1992 Jul;5(3):139-44. doi: 10.1007/BF00336598.
15-Deoxyspergualin (DSG) has been reported to be a useful immunosuppressive agent already used to inhibit acute rejection in clinical transplantation. In the present study, the survival of heart allograft in rats after a short course of DSG treatment and the mechanisms underlying DSG-induced heart allograft acceptance were studied. Male LEW rats were used as recipients. Male ACI and Wistar rats were used as donors and third-party donors, respectively. Survival of ACI heart grafts in LEW recipients treated with a short course of DSG starting on day 4 after grafting was markedly prolonged, with a mean survival time of 16.6 +/- 5.8 days and 29.8 +/- 3.0 days at doses of 2.5 mg/kg per day and 5 mg/kg per day, respectively. On day 20 after grafting, the mechanism of inducing allograft survival after DSG treatment at a dose of 5 mg/kg per day was analyzed by testing the activation of spleen cells or serum in several assay systems. Spleen cells from DSG-treated rats with surviving heart allografts showed almost no proliferative response against donor strain stimulator cells compared with controls. The cytotoxic activity towards donor strain target cells of spleen cells from DSG-treated rats with surviving heart allografts was lower than that of spleen cells from rats with rejected heart allografts.(ABSTRACT TRUNCATED AT 250 WORDS)
据报道，15-脱氧精胍菌素（DSG）是一种有效的免疫抑制剂，已用于临床移植中抑制急性排斥反应。在本研究中，研究了大鼠心脏移植在短期DSG治疗后的存活情况以及DSG诱导心脏移植接受的潜在机制。雄性LEW大鼠作为受体。雄性ACI和Wistar大鼠分别作为供体和第三方供体。在移植后第4天开始用短期DSG治疗的LEW受体中，ACI心脏移植物的存活时间显著延长，每天2.5mg/kg和5mg/kg剂量时的平均存活时间分别为16.6±5.8天和29.8±3.0天。在移植后第20天，通过在多个检测系统中检测脾细胞或血清的激活情况，分析了每天5mg/kg剂量的DSG治疗后诱导同种异体移植存活的机制。与对照组相比，心脏同种异体移植存活的DSG处理大鼠的脾细胞对供体品系刺激细胞几乎没有增殖反应。心脏同种异体移植存活的DSG处理大鼠的脾细胞对供体品系靶细胞的细胞毒性活性低于心脏同种异体移植排斥大鼠的脾细胞。（摘要截短于250字）
