Avni A, Anderson J D, Holland N, Rochaix J D, Gromet-Elhanan Z, Edelman M
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Science. 1992 Aug 28;257(5074):1245-7. doi: 10.1126/science.1387730.
Tentoxin is a naturally occurring phytotoxic peptide that causes seedling chlorosis and arrests growth in sensitive plants and algae. In vitro, it inhibits activity of the beta subunit of the plastid proton-adenosine triphosphatase (ATPase) from sensitive species. Plastid atpB genes from six closely related, tentoxin-sensitive or -resistant Nicotiana species differ at codon 83, according to their response to the toxin: glutamate correlated with resistance and aspartate correlated with sensitivity. The genetic relevance of this site was confirmed in Chlamydomonas reinhardtii by chloroplast transformation. The alga, normally tentoxin-resistant, was rendered tentoxin-sensitive by mutagenesis of its plastid atpB gene at codon 83. Codon 83 may represent a critical site on the beta subunit that does not compete with nucleotide binding or other catalytic activities.
细交链孢菌酮酸是一种天然存在的植物毒性肽，可导致敏感植物和藻类出现幼苗黄化并抑制其生长。在体外，它会抑制敏感物种质体质子 - 三磷酸腺苷酶（ATP酶）β亚基的活性。六个亲缘关系密切、对细交链孢菌酮酸敏感或抗性的烟草属物种的质体atpB基因在密码子83处存在差异，这与它们对该毒素的反应有关：谷氨酸与抗性相关，天冬氨酸与敏感性相关。通过叶绿体转化在莱茵衣藻中证实了该位点的遗传相关性。这种藻类通常对细交链孢菌酮酸具有抗性，但通过对其质体atpB基因密码子83进行诱变，使其对细交链孢菌酮酸敏感。密码子83可能代表β亚基上一个关键位点，该位点不与核苷酸结合或其他催化活性竞争。
