Selman B R, Durbin R D
Biochim Biophys Acta. 1978 Apr 11;502(1):29-37. doi: 10.1016/0005-2728(78)90128-7.
The effects of tentoxin on the ATPase activities of coupling factor 1 proteins (CF1) and photophosphorylation with isolated chloroplasts and chloroplasts reconstituted with coupling factor proteins have been examined. 1. The calcium-dependent ATPase activities of coupling factors isolated from spinach, lettuce and Nicotiana otophora are completely inhibited by tentoxin. The ATPase activities of coupling factors isolated from Nicotiana tabacum and Nicotiana knightiana are not affected by tentoxin. 2. Phenazine methosulfate-catalyzed cyclic photophosphorylation with chloroplasts isolated from spinach, lettuce and N. otophora is completely inhibited by tentoxin, whereas chloroplasts isolated from N. knightiana and N. tabacum are relatively insensitive to tentoxin. 3. Spinach chloroplasts, partially depleted in CF1, can be reconstituted with coupling factors isolated from a wide variety of plants including lettuce, radish, N. tabacum, N. knightiana and N. otophora. 4. Spinach chloroplasts reconstituted with spinach, lettuce and N. otophora CF1 retain their sensitivity to tentoxin; however, when reconstituted with N. knightiana and N. tabacum coupling factor proteins, a significant fraction of the reconstituted rate remains tentoxin insensitive. These data are interpreted as evidence that coupling factors that reconstitute with spinach thylakoid membranes have both a catalytic and structural function.
已研究了抗霉素对偶联因子1蛋白（CF1）的ATP酶活性以及对分离的叶绿体和用偶联因子蛋白重构的叶绿体的光合磷酸化作用的影响。1. 从菠菜、生菜和耳状烟草中分离的偶联因子的钙依赖性ATP酶活性被抗霉素完全抑制。从烟草和奈氏烟草中分离的偶联因子的ATP酶活性不受抗霉素影响。2. 用吩嗪硫酸甲酯催化从菠菜、生菜和耳状烟草中分离的叶绿体进行循环光合磷酸化，该过程被抗霉素完全抑制，而从奈氏烟草和烟草中分离的叶绿体对抗霉素相对不敏感。3. CF1部分缺失的菠菜叶绿体可用从多种植物（包括生菜、萝卜、烟草、奈氏烟草和耳状烟草）中分离的偶联因子进行重构。4. 用菠菜、生菜和耳状烟草CF1重构的菠菜叶绿体保持其对抗霉素的敏感性；然而，当用奈氏烟草和烟草偶联因子蛋白重构时，很大一部分重构速率对抗霉素不敏感。这些数据被解释为与菠菜类囊体膜重构的偶联因子具有催化和结构功能的证据。
