DALES S
J Cell Biol. 1962 May;13(2):303-22. doi: 10.1083/jcb.13.2.303.
Early interaction between two animal viruses, vaccinia and adenovirus 7, which multiply readily in L strain and HeLa cells, respectively, was examined in both whole mount preparations and in thin sections. To observe the association at the surface, cells carrying adsorbed virus were swelled under controlled conditions and then "stained" with neutral phosphotungstate. Each particle of both virus types becomes attached to the cell by several capsomeres and is then ingested by phagocytosis. Within the cell, near the surface, single particles or small clumps of adenovirus are lodged within vesicles. Deeper in the cytoplasm this virus is packed in large, numerous inclusions, whereas very close to the nuclear envelope only free particles are found. Vaccinia, on the other hand, either free or in vesicles, is always found in the cytoplasm, at some distance from the nucleus (11). Adsorption and intracellular disposition of these two viruses is discussed in relation to the infectious process.
在整装标本和薄片中研究了痘苗病毒和腺病毒7这两种动物病毒之间的早期相互作用，它们分别在L株细胞和HeLa细胞中易于增殖。为了观察表面的结合情况，将携带吸附病毒的细胞在可控条件下进行膨胀，然后用中性磷钨酸盐“染色”。两种病毒类型的每个颗粒都通过几个壳粒附着在细胞上，然后通过吞噬作用被摄取。在细胞内，靠近表面的地方，单个腺病毒颗粒或小团块位于小泡内。在细胞质较深处，这种病毒被包装在大量的包涵体中，而在非常靠近核膜的地方只发现游离颗粒。另一方面，痘苗病毒无论是游离的还是在小泡中的，总是存在于细胞质中，距离细胞核有一定距离（11）。结合感染过程讨论了这两种病毒的吸附和细胞内定位。
