School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.
Virol J. 2013 Jun 5;10:177. doi: 10.1186/1743-422X-10-177.
Most viruses take advantage of endocytic pathways to gain entry into host cells and initiate infections. Understanding of virus entry via endocytosis is critically important for the design of antiviral strategies. Virus entry via endocytosis is a complex process involving hundreds of cellular proteins. The entire process is dictated by events occurring at multiple time and length scales. In this review, we discuss and evaluate the available means to investigate virus endocytic entry, from both experimental and theoretical/numerical modeling fronts, and highlight the importance of multiscale features. The complexity of the process requires investigations at a systems biology level, which involves the combination of different experimental approaches, the collaboration of experimentalists and theorists across different disciplines, and the development of novel multiscale models.
大多数病毒利用内吞途径进入宿主细胞并引发感染。了解通过内吞作用的病毒进入对于设计抗病毒策略至关重要。病毒通过内吞作用进入是一个涉及数百种细胞蛋白的复杂过程。整个过程由多个时间和长度尺度上发生的事件决定。在这篇综述中，我们讨论和评估了从实验和理论/数值建模两个方面研究病毒内吞进入的现有方法，并强调了多尺度特征的重要性。该过程的复杂性需要在系统生物学水平上进行研究，这涉及到不同实验方法的结合、不同学科的实验家和理论学家的合作，以及新型多尺度模型的开发。
