EICHEL H J, ROTH J S
J Cell Biol. 1962 Feb;12(2):263-76. doi: 10.1083/jcb.12.2.263.
Some properties of rat spleen ribonuclease have been studied, and the intracellular distribution of the enzyme and ribonucleic acid have been presented. Spleen ribonuclease exhibits maximal activity at pH 5.8, and although there is some evidence for the presence of an enzyme with an optimum at pH 7.0, it is not conclusive. The enzyme is concentrated primarily in the mitochondrial fraction, but significant quantities occur in the supernatant fluid. The latter contains ribonuclease inhibitor similar to that found in liver. The effects of whole body x-irradiation, magnesium ion, substrate concentration, type of buffer, presence of p-chloromercuriphenylsulfonic acid, deoxycholate, and Triton X-100 on ribonuclease activity are examined.
对大鼠脾脏核糖核酸酶的一些特性进行了研究,并给出了该酶和核糖核酸的细胞内分布情况。脾脏核糖核酸酶在pH 5.8时表现出最大活性,虽然有一些证据表明存在一种最适pH为7.0的酶,但并不确凿。该酶主要集中在线粒体部分,但上清液中也有大量存在。后者含有与肝脏中发现的类似的核糖核酸酶抑制剂。研究了全身X射线照射、镁离子、底物浓度、缓冲液类型、对氯汞苯磺酸、脱氧胆酸盐和吐温X-100对核糖核酸酶活性的影响。