血红蛋白-肯普西稀溶液的功能行为：β99（G-1）天冬氨酸→天冬酰胺。
Functional behaviour of dilute solutions of Hb-Kempsey: beta 99 (G-1) Asp--Asn.
作者信息
Rabino Massa E, Ricco G, Polimeni C, Reddavid M
机构信息
Dip. Biologia animale, Università di Torino.
出版信息
Boll Soc Ital Biol Sper. 1992 Mar;68(3):167-74.
The authors report that a diluted solution of Hb-Kempsey, beta 99 (G-1) Asp-Asn, can be chromatographically separated from the coexistent Hb-A and functionally examined if progressively depleted in O2 by bubbling pure nitrogen in the solution. Next, at fixed times, the O2 saturations of Hb are compared with the pO2s measured. Hb-Kempsey has a p50 of 1 torr, with an n-value of 1 and a Bohr effect of -0.2. Normal Hb-A of the same patient, examined with identical methods, presents: p50 = 4.5 torr; n = 2.7; Bohr effect = -0.412. Therefore, Hb-Kempsey is strongly hyperaffinic, does not display any heme-heme interaction, and has a half-normal Bohr effect.
作者报告称，Hb-Kempsey的稀释溶液，即β99（G-1）天冬氨酸-天冬酰胺，可以通过色谱法与共存的Hb-A分离，并且如果通过在溶液中鼓入纯氮气使其逐渐耗尽氧气，就可以对其进行功能检测。接下来，在固定时间，将Hb的氧饱和度与测量的pO2进行比较。Hb-Kempsey的p50为1托，n值为1，玻尔效应为-0.2。用相同方法检测的同一患者的正常Hb-A呈现：p50 = 4.5托；n = 2.7；玻尔效应 = -0.412。因此，Hb-Kempsey具有很强的高亲和力，不显示任何血红素-血红素相互作用，并且具有正常玻尔效应的一半。
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