Rabino-Massa E, Polimeni C, Reddavid M, Ricco G
Dip. Biologia animale, Università di Torino.
Boll Soc Ital Biol Sper. 1992 Mar;68(3):159-66.
The authors found that, by continuously bubbling pure nitrogen within a dilute Hb solution, the latter releases its oxygen with an intensity which is quite proportional to the duration of bubbling. Such a procedure can be employed instead of the common tonometric technique, to measure functional properties of Hb. In fact, not only dissociation curves can be obtained, but also a correct evaluation of the affinity changes upon pH variations (Bohr effect). The results obtained by this technique are not far from those recently reported by using tonometry, although the method is less precise and still not suitable for routine purposes. Nevertheless, it can be substantially improved.
作者发现，通过在稀释的血红蛋白溶液中持续通入纯氮气，后者释放氧气的强度与鼓泡持续时间成正比。这种方法可用于替代常规的测压技术，以测量血红蛋白的功能特性。实际上，不仅可以获得解离曲线，还能正确评估pH变化时亲和力的变化（波尔效应）。尽管该技术不如测压法精确，仍不适用于常规用途，但通过该技术获得的结果与最近使用测压法报告的结果相差不大。不过，它仍可得到实质性改进。
