Ricco G, David O, Petroni D, Cilli V, Rosso C, Rabino-Massa E
Department of Biomedical Sciences and Human Oncology, S. Luigi Gonzaga Hospital, Orbassano (Turin), Italy.
Panminerva Med. 1997 Jun;39(2):153-7.
Dissociation curves for oxygen of dilute samples of human adult Hb-A were evaluated on this occasion, by using the Oximeter-539 WTW with its sensor, and a suitable spectrophotometer. At this purpose, Hb samples were desaturated in oxygen upon given experimental conditions, by bubbling pure nitrogen in them, and their re-oxigenation in air was followed, step by step, by multiple oximetries. In addition, all the spectrophotometric measurements of the saturation of Hb-O2%, corresponding to each individual oximetry, were carried out parallely but separately. Dilution of Hb-A was maintained at 0.1 mM in heme. The p50 at pH 7.3 was 4.435 +/- 0.299 Torr, with the n-value of 2.7 +/- 0.2; Bohr effect was -0.55 +/- 0.08, within a pH range between 6.8, 7.3 and 7.8, whereas chloride and DPG effects at pH 7.3 (the most useful value) were 0.42 +/- 0.44 and 0.453 +/- 0.0187 respectively. In conclusion, these results are similar to those obtained with automated procedures, upon comparable experimental conditions, but do not require expensive and sophisticated instruments. Such a technique could be very useful in the hemoglobinopathies, which are common in Italy, and it could be easily adapted to perform comparative studies on animal hemoglobins not far from human species.
此次，使用配备传感器的WTW Oximeter - 539型血氧计以及一台合适的分光光度计，对成人稀释血红蛋白A（Hb - A）样本的氧解离曲线进行了评估。为此，在给定的实验条件下，通过向样本中鼓入纯氮气使Hb样本中的氧去饱和，然后通过多次血氧测定逐步跟踪其在空气中的再氧合过程。此外，与每次单独的血氧测定相对应的Hb - O₂饱和度的所有分光光度测量均并行但分开进行。Hb - A的稀释度维持在血红素浓度为0.1 mM。在pH 7.3时，p50为4.435±0.299托，n值为2.7±0.2；在pH值介于6.8、7.3和7.8之间时，玻尔效应为 - 0.55±0.08，而在pH 7.3（最有用的值）时，氯离子和二磷酸甘油酸（DPG）效应分别为0.42±0.44和0.453±0.0187。总之，可以看出，在可比的实验条件下，这些结果与通过自动化程序获得的结果相似，但不需要昂贵且复杂的仪器。这种技术在意大利常见的血红蛋白病中可能非常有用，并且可以很容易地适用于对与人类相近的动物血红蛋白进行比较研究。
