UV absorption complicates PCR decontamination.
作者信息
Frothingham R, Blitchington R B, Lee D H, Greene R C, Wilson K H
机构信息
Duke University Medical Center, Durham, NC 27710.
出版信息
Biotechniques. 1992 Aug;13(2):208-10.
PMID:1389153
Abstract
UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.
摘要
Zotero 插件