Inhibition of PCR by mineral oil exposed to UV irradiation for prolonged periods.

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超能文献

作者信息

Dohner D E, Dehner M S, Gelb L D

机构信息

VA Medical Center, Medical Service, St. Louis, MO 63106, USA.

出版信息

Biotechniques. 1995 Jun;18(6):964-7.

PMID:7546716

Abstract

摘要

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Inhibition of PCR by mineral oil exposed to UV irradiation for prolonged periods.

Biotechniques. 1995 Jun;18(6):964-7.

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Automated "hot start" PCR using mineral oil and paraffin wax.

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UV light irradiation of plastic reaction tubes inhibits PCR.

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Increased PCR sensitivity by using paraffin wax as a reaction mix overlay.

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Easy removal of oil from microtiter dishes following PCR.

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引用本文的文献

Inhibition and facilitation of nucleic acid amplification.

Appl Environ Microbiol. 1997 Oct;63(10):3741-51. doi: 10.1128/aem.63.10.3741-3751.1997.

Hydroxyquinoline overcomes PCR inhibition by UV-damaged mineral oil.

Nucleic Acids Res. 1995 Oct 11;23(19):4001-2. doi: 10.1093/nar/23.19.4001.

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相似文献

Inhibition of PCR by mineral oil exposed to UV irradiation for prolonged periods.

Biotechniques. 1995 Jun;18(6):964-7.

Use of UV irradiation to reduce false positivity in polymerase chain reaction.

Biotechniques. 1991 Apr;10(4):442, 444, 446.

Hydroxyquinoline overcomes PCR inhibition by UV-damaged mineral oil.

Nucleic Acids Res. 1995 Oct 11;23(19):4001-2. doi: 10.1093/nar/23.19.4001.

UV absorption complicates PCR decontamination.

Biotechniques. 1992 Aug;13(2):208-10.

Profound inhibition of the PCR step of CF V3 multiplex PCR/OLA assay by the use of UV-irradiated plastic reaction tubes.

Diagn Mol Pathol. 2007 Jun;16(2):121-3. doi: 10.1097/PDM.0b013e31803071b9.

Automated "hot start" PCR using mineral oil and paraffin wax.

Biotechniques. 1993 Jan;14(1):30-4.

A method of using heavy mineral oil for performing "hot-start" amplification of rare nucleic acids.

Anal Biochem. 1994 Aug 15;221(1):210-2. doi: 10.1006/abio.1994.1402.

UV light irradiation of plastic reaction tubes inhibits PCR.

Biotechniques. 1999 Aug;27(2):252, 254-4, 256. doi: 10.2144/99272bm07.

Increased PCR sensitivity by using paraffin wax as a reaction mix overlay.

Mol Cell Probes. 1993 Jun;7(3):249-52. doi: 10.1006/mcpr.1993.1036.

Easy removal of oil from microtiter dishes following PCR.

Biotechniques. 1991 Aug;11(2):185.

引用本文的文献

Inhibition and facilitation of nucleic acid amplification.

Appl Environ Microbiol. 1997 Oct;63(10):3741-51. doi: 10.1128/aem.63.10.3741-3751.1997.

Hydroxyquinoline overcomes PCR inhibition by UV-damaged mineral oil.

Nucleic Acids Res. 1995 Oct 11;23(19):4001-2. doi: 10.1093/nar/23.19.4001.