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Presence of single-stranded DNA in PCR products of slow electrophoretic mobility.

作者信息

Valentine J E, Boyle M J, Sewell W A, Gough N M

机构信息

Centre for Immunology St. Vincent's Hospital, Sydney NSW, Australia.

出版信息

Biotechniques. 1992 Aug;13(2):222-4.

PMID:1389155
Abstract

PCR products were characterized by electrophoresis, blotting and hybridization. In addition to the bands of expected size, bands of slower electrophoretic mobility were often detected. The slower bands completely disappeared when the PCR products were subjected to slow cooling, treated with S1 nuclease or run on an alkaline gel, whereas the bands of expected size were unaffected. The slower bands are therefore likely to contain single-stranded DNA.

摘要

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