Hookey J V, Birtles R J, Saunders N A
Division of Microbiological Reagents, Central Public Health Laboratory, Colindale, London, United Kingdom.
J Clin Microbiol. 1995 Sep;33(9):2377-81. doi: 10.1128/jcm.33.9.2377-2381.1995.
A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions was developed for the identification of species within the family Legionellaceae. The sizes of the PCR products varied from 1,353 to 350 bp. Strains of Legionella pneumophila were characterized as having products of approximately 900 and 530 bp, and L. birminghamensis had products of 1,390, 960, and 380 bp. Of the 38 species of legionellae examined, only 7 were indistinguishable (L. erythra from L. rubrilucens, L. anisa or L. cherrii from L. tucsonensis, and L. quateirensis from L. shakespearei). Two environmental isolates were identified as L. pneumophila. Strain LLAP-3, which was a symbiont of amoebae, could not be associated with any Legionella sp. studied.
开发了一种基于聚合酶链反应（PCR）扩增16S核糖体RNA基因（rDNA）-23S rDNA基因间隔区的方法，用于嗜肺军团菌科内物种的鉴定。PCR产物的大小在1353至350碱基对之间变化。嗜肺军团菌菌株的特征是具有约900和530碱基对的产物，而伯明翰军团菌具有1390、960和380碱基对的产物。在所检测的38种军团菌中，只有7种无法区分（红军团菌与微红军团菌、图森军团菌中的anis a军团菌或cherrii军团菌、莎士比亚军团菌中的夸特尔军团菌）。两个环境分离株被鉴定为嗜肺军团菌。菌株LLAP-3是变形虫的共生体，与所研究的任何军团菌都没有关联。
