Harline M C, Kandala J C, Sage R D, Guntaka R V, DeAngelo A
Department of Molecular Microbiology and Immunology, University of Missouri-Columbia 65212.
Biotechniques. 1992 Sep;13(3):388-91.
The ability to successfully screen a lambda gt11 cDNA expression library for specific gene products that can bind to selected sequences of DNA depends on radioactive double-stranded DNA probes with high specific activity. We demonstrate here that probes labeled by the PCR are superior to probes made by the Klenow reaction. The use of these PCR-generated probes have facilitated our efforts to isolate recombinant phage containing putative DNA-binding gene products that recognized a 246-base pair transcriptional enhancer region of Rous sarcoma virus long terminal repeat.
