聚合酶链反应在分析重组cDNA克隆中的便捷应用。
Convenient uses of polymerase chain reaction in analyzing recombinant cDNA clones.
作者信息
Nishikawa B K, Fowlkes D M, Kay B K
机构信息
Dept. of Biology, University of North Carolina, Chapel Hill 27599-3280.
出版信息
Biotechniques. 1989 Jul-Aug;7(7):730-5.
PMID:2534353
Abstract
We have used polymerase chain reaction to accelerate our analysis of recombinant lambda-cDNA clones. We have amplified the inserts of lambda gt10 or lambda gt11 recombinants starting with bacteriophage in cored plaques or isolated DNA. The amplifications made with simple or complex oligonucleotide primers have allowed convenient sizing, subcloning and translation of the phage inserts into protein.
摘要
我们已使用聚合酶链反应来加速对重组λ-cDNA克隆的分析。我们从有核心噬菌斑中的噬菌体或分离的DNA开始，扩增了λgt10或λgt11重组体的插入片段。用简单或复杂的寡核苷酸引物进行的扩增，使得对噬菌体插入片段进行方便的大小测定、亚克隆以及将其翻译成蛋白质成为可能。
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