脱氧核糖核酸的声波降解研究。
Studies on the sonic degradation of deoxyribonucleic acid.
作者信息
FREIFELDER D, DAVISON P F
出版信息
Biophys J. 1962 May;2(3):235-47. doi: 10.1016/s0006-3495(62)86852-0.
Abstract
T7 DNA was partially degraded by x-rays, DNAase, and sonic irradiation. The molecular weight distributions were calculated from sedimentation velocity studies on the resulting preparations. Comparison with the theoretical curve derived by Montroll and Simha showed that the first two degradative methods act grossly at random, whereas sonication is a non-random process resulting in the preferential halving of the DNA molecules in solution.
摘要
T7噬菌体DNA被X射线、脱氧核糖核酸酶和超声辐射部分降解。通过对所得制剂进行沉降速度研究来计算分子量分布。与蒙特罗尔和西姆哈推导的理论曲线比较表明，前两种降解方法大致随机起作用，而超声处理是一个非随机过程，导致溶液中的DNA分子优先减半。
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