Silhánková L, Málková-Kadlecová Z, Studlarová Z
Folia Microbiol (Praha). 1978;23(3):216-24. doi: 10.1007/BF02876582.
Ultrasonication at 20 kHz, intensity 35 W/cm2 and amplitude 15--25 micron of a diploid strain of Saccharomyces cerevisiae was found to act as a weak mutagen with maximum efficiency at the 20% survival of the cells. Under these conditions, the frequency of reversion of the suppressible allel ilv1-92 increased ten times, the frequency of mitotic gene conversion four times. Doses leading to survivals lower than 20% led to a slight increase in the frequency of cytoplasmic respiration-deficient mutants. Submutagenic doses applied immediately after gammaradiation or UV light did not substantially increase the effect of these physical agents on the genetic material of the yeast strain investigated. Application of ultrasound prior to UV radiation did not considerably influence the effect of the radiation either.
研究发现，对酿酒酵母二倍体菌株进行20千赫兹、强度35瓦/平方厘米、振幅15 - 25微米的超声处理，在细胞存活率为20%时，其作为一种弱诱变剂的效率最高。在这些条件下，可抑制等位基因ilv1 - 92的回复突变频率增加了10倍，有丝分裂基因转换频率增加了4倍。导致存活率低于20%的剂量会使细胞质呼吸缺陷型突变体的频率略有增加。在γ射线或紫外线照射后立即施加亚诱变剂量，并不会显著增加这些物理因素对所研究酵母菌株遗传物质的影响。在紫外线辐射之前施加超声波，对辐射效果也没有太大影响。
