Kelley W D
Can J Microbiol. 1975 Oct;21(10):1548-52. doi: 10.1139/m75-227.
Significant differences in amylase, beta-glucosidase, and phosphatase activities were observed among four Phytophthora cinnamomi isolates grown in nutrient-amended sterilized soil for 20 days. Amylase pH optima for the four isolates were within a relatively narrow range; at pH 5.5 each isolate was within 90% of its peak activity. Isolates SB-216-1, 1-281, and C-39 each exhibited maximal beta-glucosidase activity at pH 5.0 and maximal phosphatase activity at pH 5.0-5.5. Maximal activity for these two enzymes of isolate A-7725 occurred at pH 3.5. In timed experiments, isolates 1-281 and A-7725 exhibited greater amylase activities than did the other two isolates. For beta-glucosidase, greatest activity was observed for SB-216-1; ACTivity of 1-281 was intermediate and least activity was observed for isolates A-7725 and C-39. Isolates SB-216-1 and 1-281 exhibited greatest phosphatase activities; isolate C-39 was intermediate in activity, and A-7725 was least active. Results indicate that significant differences exist among the isolates tested and that these differences can be quantitatively measured by the methods described.
在营养改良的灭菌土壤中培养20天的四种樟疫霉菌株之间，观察到淀粉酶、β-葡萄糖苷酶和磷酸酶活性存在显著差异。这四种菌株的淀粉酶最适pH值在相对较窄的范围内；在pH 5.5时，每种菌株的活性均在其峰值活性的90%以内。菌株SB-216-1、1-281和C-39在pH 5.0时均表现出最大的β-葡萄糖苷酶活性，在pH 5.0 - 5.5时表现出最大的磷酸酶活性。菌株A-7725的这两种酶的最大活性出现在pH 3.5时。在定时实验中，菌株1-281和A-7725表现出比其他两种菌株更高的淀粉酶活性。对于β-葡萄糖苷酶，观察到SB-216-1的活性最高；1-281的活性处于中等水平，而A-7725和C-39的活性最低。菌株SB-216-1和1-281表现出最高的磷酸酶活性；菌株C-39的活性处于中等水平，而A-7725的活性最低。结果表明，所测试的菌株之间存在显著差异，并且这些差异可以通过所述方法进行定量测量。
