Zbieranowski I, Le Riche J C, Palcic B, Gascoyne R, Connors J
Division of Cytology, British Columbia Cancer Agency, Vancouver Clinic, Canada.
Anal Cell Pathol. 1992 Jul;4(4):303-13.
Paraffin-embedded tissue from a series of 40 cases of diffuse, large cell lymphoma was analyzed by both flow and image cytometry to compare the ability of these techniques to detect DNA aneuploid populations. Image cytometry (ICM) was performed both on nuclear suspensions and tissue sections. Twenty cases (50%) were non-diploid by at least one method of analysis. Twenty-five percent of the cases were aneuploid by flow cytometry (FCM) alone. The majority of these cases were near-diploid tumors which could not be resolved by ICM. Peri-tetraploid peaks were identified by ICM of tissue sections alone in 15% of the cases. There was an apparent loss of these peri-tetraploid cells during the preparation of the nuclear suspensions. The remaining cases showed a good correlation between all three methods in the determination of DNA ploidy. Flow and image cytometry are complimentary techniques when applied to archival tissue, however aneuploid populations may be missed if ICM is not performed on tissue sections.
对40例弥漫性大细胞淋巴瘤的石蜡包埋组织进行了流式细胞术和图像细胞术分析，以比较这些技术检测DNA非整倍体群体的能力。图像细胞术（ICM）在核悬液和组织切片上均进行了检测。至少一种分析方法显示20例（50%）为非二倍体。25%的病例仅通过流式细胞术（FCM）检测为非整倍体。这些病例中的大多数是近二倍体肿瘤，无法通过ICM分辨。仅在15%的病例中，通过组织切片的ICM鉴定出了近四倍体峰。在制备核悬液过程中，这些近四倍体细胞明显丢失。其余病例在DNA倍性测定中，三种方法之间具有良好的相关性。当应用于存档组织时，流式细胞术和图像细胞术是互补技术，然而，如果不对组织切片进行ICM检测，可能会遗漏非整倍体群体。
