Wilson J L, Dollard S C, Chow L T, Broker T R
Department of Biochemistry, University of Rochester, School of Medicine and Dentistry, New York 14642.
Cell Growth Differ. 1992 Aug;3(8):471-83.
Cultured epithelial cells are used to generate extensive patches of autologous skin equivalent for patients with burns or wounds and to investigate the growth and differentiation of epithelia in vitro. We have undertaken a comprehensive study of the morphological and molecular events that occur during culturing of human foreskin keratinocytes at the liquid-air interface on a dermal equivalent consisting of a collagen matrix containing fibroblasts. Using radioactively labeled RNA probes for mRNAs and monoclonal antibodies for proteins, we found that the expression of a comprehensive set of differentiation stage-specific genes was affected by the type of fibroblasts included in the matrix as well as by the age of the culture. The expression of these genes was not always coordinated and could not be predicted from the histological appearance of the stratified epithelium. Surprisingly, the mouse fibroblasts promoted epithelial differentiation much more closely resembling foreskin than did the homologous primary foreskin fibroblasts.
培养的上皮细胞可用于为烧伤或伤口患者生成大面积的自体皮肤替代物，并用于体外研究上皮细胞的生长和分化。我们对人包皮角质形成细胞在含有成纤维细胞的胶原基质真皮替代物上的液-气界面培养过程中发生的形态学和分子事件进行了全面研究。使用针对mRNA的放射性标记RNA探针和针对蛋白质的单克隆抗体，我们发现一组全面的分化阶段特异性基因的表达受基质中所含成纤维细胞类型以及培养时间的影响。这些基因的表达并不总是协调一致的，也无法从分层上皮的组织学外观预测。令人惊讶的是，与同源的原代包皮成纤维细胞相比，小鼠成纤维细胞更能促进上皮细胞分化，使其更类似于包皮。
