Ning J, Peterson L M, Anderson P J, Biro G P
Department of Physiology and Biochemistry, University of Ottawa, Ontario, Canada.
Biomater Artif Cells Immobilization Biotechnol. 1992;20(2-4):723-7. doi: 10.3109/10731199209119709.
Isovolumic exchange transfusion (25% of total estimated blood volume) was carried out in the anesthetized dogs using 9 g/dl of unmodified human stroma-free hemoglobin solution (SFHS). The objective was to determine the systemic hemodynamic, blood distribution and renal effects of SFHS over a 2-3 hour period post-exchange. At 30 minutes after the exchange, blood pressure rose from 114 +/- 117 to 133 +/- 22 mmHg, but this rise was not sustained thereafter. Mean pulmonary arterial blood pressure rose from 8 +/- 3 to 13 +/- 2 mmHg, and remained above the pre-exchange level up to 3 hours post-exchange. Cardiac output remained within normal limits. Significant flow-increments were seen at 30 minutes in heart, brain, liver, gut, and kidney, but these were also not sustained. A fall in glomerular filtration rate (GFR) occurred after the exchange and remained below the pre-exchange level. A reduction in urine flow at 150 minutes post-exchange was observed and was accompanied by a reduction in urinary electrolyte excretion. The findings suggest that the initial effects of the administration of unmodified stroma-free hemoglobin solution are those of peripheral vasoconstriction which does not appear to significantly restrict flow to the vital organs, such as heart and brain. Unmodified hemoglobin was found to cause a decrease in renal function.
在麻醉犬中使用9g/dl未修饰的人无基质血红蛋白溶液（SFHS）进行等容交换输血（占估计总血容量的25%）。目的是确定交换后2至3小时内SFHS对全身血流动力学、血液分布和肾脏的影响。交换后30分钟，血压从114±117mmHg升至133±22mmHg，但此后这种升高并未持续。平均肺动脉血压从8±3mmHg升至13±2mmHg，并在交换后3小时内一直高于交换前水平。心输出量保持在正常范围内。在30分钟时，心脏、脑、肝、肠和肾脏出现显著的血流增加，但这些也未持续。交换后肾小球滤过率（GFR）下降，并一直低于交换前水平。在交换后150分钟观察到尿流减少，并伴有尿电解质排泄减少。这些发现表明，给予未修饰的无基质血红蛋白溶液的初始作用是外周血管收缩，这似乎并未显著限制流向心脏和脑等重要器官的血流。未修饰的血红蛋白被发现会导致肾功能下降。
