Amend J, Ou C, Ryan-MacFarlane C, Anderson P J, Amend N, Biro G P
Dept. of Physiology, University of Ottawa, ON, Canada.
Artif Cells Blood Substit Immobil Biotechnol. 1996 Jan;24(1):19-34. doi: 10.3109/10731199609117428.
Anesthetized mongrel (weight range: 16-27 Kg) dogs were prepared for monitoring hemodynamics, blood flow distribution, plasma colloid osmotic pressure and renal functional parameters at various intervals. Removal of 35 ml/Kg blood resulted in marked drop and only partial spontaneous recovery in systemic and pulmonary arterial pressures, cardiac output and organ blood flows (> 50% flow-decrements occurred in kidney, spleen, heart, gut and pancreas); plasma colloid osmotic pressure as well as urine output and creatinine clearance also fell. Group I (n = 6) of dogs was transfused after 45 minutes of hypovolemia with their own anticoagulated blood, while Group II (n = 6) received an equal volume of unmodified 6% stromafree hemoglobin solution (SFHS). Comparison of the two groups' responses to resuscitation yielded some differences. There was a significant overshoot (30 mmHg) in systemic arterial blood pressure accompanied by bradycardia in Group II only. Cardiac output recovered in both groups but was less well sustained in Group II. Cerebral blood flow rose higher and hepatic arterial flow-increment was less in Group II than in Group I; the responses to resuscitation in the other organs were comparable. Colloid osmotic pressure decreased in Group I whereas it rose immediately after resuscitation in Group II, declining thereafter with a converging trend and 30 minutes thereafter the differences were not significant between the groups. Urine excretion and creatinine clearance recovered to comparable extents in both groups, but N-acetyl-beta-D-glucosaminidase (N.A.G.) excretion rose over 10-fold higher in Group II than in Group I. These experiments have defined the response of bled animals to resuscitation with unmodified, unpurified SFHS, when compared to resuscitation with whole blood, showing a less well sustained but adequate hemodynamic and renal functional recovery while revealing indications of early renal tubular cellular injury, providing baseline comparison for testing highly purified and modified hemoglobin solutions.
对麻醉的杂种犬（体重范围：16 - 27千克）进行准备，以便在不同时间间隔监测血流动力学、血流分布、血浆胶体渗透压和肾功能参数。抽取35毫升/千克血液导致全身和肺动脉压、心输出量以及器官血流量显著下降，且仅部分自发恢复（肾脏、脾脏、心脏、肠道和胰腺的血流量减少超过50%）；血浆胶体渗透压以及尿量和肌酐清除率也下降。第一组（n = 6）犬在低血容量45分钟后输注自身抗凝血液，而第二组（n = 6）接受等量未修饰的6%无基质血红蛋白溶液（SFHS）。比较两组复苏反应产生了一些差异。仅第二组出现全身动脉血压显著超调（30 mmHg）并伴有心动过缓。两组的心输出量均恢复，但第二组维持得较差。第二组脑血流量升高幅度更大，肝动脉血流增量比第一组小；其他器官的复苏反应相当。第一组胶体渗透压降低，而第二组复苏后立即升高，此后下降且呈收敛趋势，30分钟后两组间差异不显著。两组的尿排泄和肌酐清除率恢复程度相当，但第二组N - 乙酰 - β - D - 氨基葡萄糖苷酶（N.A.G.）排泄量比第一组高出10倍以上。这些实验确定了失血动物与输注全血相比，用未修饰、未纯化的SFHS复苏的反应，显示出维持效果较差但足够的血流动力学和肾功能恢复，同时揭示了早期肾小管细胞损伤的迹象，为测试高度纯化和修饰的血红蛋白溶液提供了基线比较。
