Chen C G, Cornish E C, Clarke A E
Plant Cell Biology Research Centre, University of Melbourne, Parkville, Victoria, Australia.
Plant Cell. 1992 Sep;4(9):1053-62. doi: 10.1105/tpc.4.9.1053.
cDNAs and corresponding genomic clones encoding a putative proline-rich protein (NaPRP3) were isolated from libraries prepared from Nicotiana alata style mRNA and genomic DNA. The predicted NaPRP3 protein is structurally similar to extensin in containing six copies of the characteristic extensin sequence Ser-Pro4, but differs in being smaller (151 residues compared with greater than 300 residues) and lacking Tyr residues. In contrast to most extensin genes, the NaPRP3 gene is not induced by mechanical wounding, and its expression is restricted to cells of the transmitting tract of the style.
从烟草花柱mRNA和基因组DNA构建的文库中分离出了编码一种假定富含脯氨酸蛋白(NaPRP3)的cDNA和相应的基因组克隆。预测的NaPRP3蛋白在结构上与伸展蛋白相似,含有六个特征性的伸展蛋白序列Ser-Pro4拷贝,但不同之处在于其较小(151个残基,而伸展蛋白大于300个残基)且缺乏酪氨酸残基。与大多数伸展蛋白基因不同,NaPRP3基因不会被机械损伤诱导,其表达仅限于花柱传递组织的细胞。