von Heijne G
Eur J Biochem. 1983 Jun 1;133(1):17-21. doi: 10.1111/j.1432-1033.1983.tb07424.x.
According to the signal hypothesis, a signal sequence, once having initiated export of a growing protein chain across the rough endoplasmic reticulum, is cleaved from the mature protein at a specific site. It has long been known that some part of the cleavage specificity resides in the last residue of the signal sequence, which invariably is one with a small, uncharged side-chain, but no further specific patterns of amino acids near the point of cleavage have been discovered so far. In this paper, some such patterns, based on a sample of 78 eukaryotic signal sequences, are presented and discussed, and a first attempt at formulating rules for the prediction of cleavage sites is made.
根据信号假说，信号序列一旦启动正在生长的蛋白质链穿过糙面内质网的输出过程，就会在特定位点从成熟蛋白质上裂解下来。长期以来人们一直知道，裂解特异性的某些部分存在于信号序列的最后一个残基中，该残基总是带有小的不带电荷侧链的氨基酸，但到目前为止，尚未发现裂解点附近氨基酸的进一步特定模式。在本文中，基于78个真核生物信号序列的样本，展示并讨论了一些此类模式，并首次尝试制定预测裂解位点的规则。
