POLAK M F, BRANS L M, BEUNDERSB J, van der WERFF A
Bull World Health Organ. 1962;27(3):311-6.
In view of doubts as to the potency level required to obtain nearly 100% successful vaccinations with the glycerolated smallpox vaccine prepared by the Netherlands National Institute of Public Health, its dose-effect relationship was compared with that of a glycerolated lymph from the Lister Institute of Preventive Medicine, England. The potency was estimated from pock counts on the chorio-allantoic membrane of chick embryos and the effect was judged from the "take" rate obtained in the primary vaccination of soldiers, the data being subjected to probit analysis.The relationship observed between the number of pock-forming units and the percentage of successful primary vaccinations was nearly identical with the two vaccines. The 50% effective doses were found to be 1.3-1.4 x 10(6) PFU/ml and the 99% doses to be 4.1-4.3 x 10(7) PFU/ml.
鉴于对荷兰国家公共卫生研究所制备的甘油化天花疫苗获得近100%成功接种所需效价水平存在疑问，将其剂量效应关系与英国李斯特预防医学研究所的甘油化痘苗进行了比较。通过鸡胚绒毛尿囊膜上的痘疱计数来估计效价，并根据士兵初次接种的“接种”率来判断效果，数据进行了概率分析。两种疫苗在形成痘疱单位数量与初次接种成功百分比之间观察到的关系几乎相同。发现50%有效剂量为1.3 - 1.4×10⁶PFU/ml，99%剂量为4.1 - 4.3×10⁷PFU/ml。
