Waanders G A, Hugo P, Boyd R L
Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria, Australia.
Cell Immunol. 1992 Oct 1;144(1):237-45. doi: 10.1016/0008-8749(92)90240-p.
Using fetal thymic organ culture (FTOC), we describe the effects of IL-1 on T cell differentiation, particularly within the CD4-CD8- subset. While treatment of FTOC with IL-1 led to a modest reduction in total thymocyte yield, it induced an increase in the percentage of CD4-CD8- cells that express IL-2R early in culture and a decrease in the number of their precursors (CD44+IL-2R- cells). The increase in the percentage of cells expressing IL-2R was not accompanied by an increase in the number of these cells. At later time points these IL-2R+ cells (and their precursors) were reduced relative to controls. The total number of CD4-CD8-CD3- precursor cells in IL-1-treated cultures was reduced to approximately half that in controls at Day 12 of culture. However, only minor inhibition of total cell number was observed, which, taken together with the greater frequency of IL-2R+ precursors, suggests that this depletion of the pool of precursors may have been due to the induction of premature differentiation rather than to its inhibition.
利用胎儿胸腺器官培养（FTOC），我们描述了白细胞介素-1（IL-1）对T细胞分化的影响，特别是在CD4-CD8-亚群内的影响。虽然用IL-1处理FTOC导致胸腺细胞总产量略有下降，但它诱导培养早期表达IL-2R的CD4-CD8-细胞百分比增加，而其前体细胞（CD44+IL-2R-细胞）数量减少。表达IL-2R的细胞百分比增加并未伴随着这些细胞数量的增加。在后期时间点，相对于对照，这些IL-2R+细胞（及其前体细胞）减少。在培养第12天，用IL-1处理的培养物中CD4-CD8-CD3-前体细胞的总数减少至对照的约一半。然而，仅观察到总细胞数量的轻微抑制，这与IL-2R+前体细胞的更高频率一起表明，前体细胞池的这种消耗可能是由于诱导了过早分化而非抑制分化所致。
