Small M, Weissman I L
Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
Scand J Immunol. 1996 Aug;44(2):115-21. doi: 10.1046/j.1365-3083.1996.d01-287.x.
CD3-4-8- interleukin-2 receptor positive (IL-2R+) thymocyte precursors from adult mice were cocultured with thymic stromal cells from syngeneic adult mice. The IL-2R+CD3-4-8- thymocytes were obtained by positive panning of IL-2R+ cells followed by either sorting or negative panning of triple negative cells, and they were cocultured with primary or secondary cultures of heterogeneous thymic stromal cells. Phenotypic maturation of these precursor cells was extremely rapid. Within 2 1/2 days significant numbers of CD4+8+ and CD3+4+8- cell populations developed, the latter expressing the alpha beta T-cell receptor (alpha beta-TCR). Thus heterogeneous stromal cell cultures support the development of IL-2R+ precursors and with these methods it will now be possible to isolate the particular stromal cells involved at each stromal-dependent step.
将成年小鼠的CD3 - 4 - 8 - 白细胞介素2受体阳性（IL - 2R +）胸腺细胞前体与同基因成年小鼠的胸腺基质细胞进行共培养。通过对IL - 2R +细胞进行阳性淘选，然后对三阴性细胞进行分选或阴性淘选获得IL - 2R + CD3 - 4 - 8 - 胸腺细胞，并将它们与异质性胸腺基质细胞的原代或传代培养物进行共培养。这些前体细胞的表型成熟极其迅速。在2.5天内，大量的CD4 + 8 +和CD3 + 4 + 8 - 细胞群体形成，后者表达αβT细胞受体（αβ - TCR）。因此，异质性基质细胞培养支持IL - 2R +前体的发育，通过这些方法现在有可能分离出在每个基质依赖性步骤中涉及的特定基质细胞。
