Theis G A
J Immunol. 1977 Mar;118(3):887-94.
PHA responses have been measured in lymphoid cell cultures prepared by mixing normal chicken spleen cells with spleen or thymus cells from syngeneic chickens infected with the oncogenic herpesvirus MDV. Results of these studies may be summarized as follows: 1) spleen cells from MDV-infected chickens with visceral lymphomas inhibit the PHA response of normal spleen cells possibly by release of soluble inhibitory factors in response to the mitogen; 2) lymphoid cells from asymptomatic MDV-infected chickens, although hyporeactive themselves to PHA, can have a stimulatory effect on PHA responses of normal spleen cells in mixed cultures; 3) spleen cells from MDV-infected chickens, effectively protected from viral oncogenesis by HVT vaccination, show normal reactivity to PHA in spearate cultures and may react in mixed cultures like normal lymphocytes, with neither a pronounced stimulatory nor inhibitory effect on the PHA response of normal spleen cells.
已通过将正常鸡脾脏细胞与感染致癌性疱疹病毒MDV的同基因鸡的脾脏或胸腺细胞混合制备淋巴细胞培养物，来测量PHA反应。这些研究结果可总结如下：1）患有内脏淋巴瘤的MDV感染鸡的脾脏细胞可能通过释放可溶性抑制因子来响应有丝分裂原，从而抑制正常脾脏细胞的PHA反应；2）无症状MDV感染鸡的淋巴细胞虽然自身对PHA反应低下，但在混合培养物中可对正常脾脏细胞的PHA反应产生刺激作用；3）通过HVT疫苗有效预防病毒致癌的MDV感染鸡的脾脏细胞，在单独培养物中对PHA表现出正常反应性，并且在混合培养物中可能像正常淋巴细胞一样反应，对正常脾脏细胞的PHA反应既无明显刺激作用也无抑制作用。
