Pan Y X
Beijing Institute of Tuberculosis.
Zhonghua Jie He He Hu Xi Za Zhi. 1992 Feb;15(1):36-7, 62.
The whole chromosomal DNA probes prepared from M. tuberculosis and M. intracellulare were used to hybridize in DNA dot blot and bacterial dot blot hybridization with 9 reference strains of Mycobacteria, including M. tuberculosis, M. bovis, M. avium, M. intracellulare, M kansasii, etc. These two probes showed good sensitivity and specificity for M. tuberculosis complex and M. avium complex respectively. Using the colony hybridization in situ, we can detect and identify M. tuberculosis with the probes on NC membrane after 1-2 week incubation over 7H9 agar plate.
用结核分枝杆菌和胞内分枝杆菌制备的全染色体DNA探针，与9株分枝杆菌参考菌株，包括结核分枝杆菌、牛分枝杆菌、鸟分枝杆菌、胞内分枝杆菌、堪萨斯分枝杆菌等，进行DNA斑点杂交和细菌斑点杂交。这两种探针分别对结核分枝杆菌复合群和鸟分枝杆菌复合群显示出良好的敏感性和特异性。通过原位菌落杂交，在7H9琼脂平板上培养1-2周后，我们可以用NC膜上的探针检测和鉴定结核分枝杆菌。
