Reddi P P, Talwar G P, Khandekar P S
National Institute of Immunology, New Delhi.
Indian J Biochem Biophys. 1991 Aug;28(4):243-6.
A recombinant genomic clone was isolated from a lambda gt 11 library of M. tuberculosis on the basis of lack of hybridization with M. avium and M. kansasi. The specificity and sensitivity of M. tb DNA probes, 2.5 and 2.3 kb in size, were assessed by Southern blot and dot blot hybridization. These did not cross hybridize to DNA of mycobacteria other than members of M. tb complex, nor with DNA of non mycobacterial origin. Sensitivity was determined to be 200 pg which is equivalent to 10(4) bacilli. Genomic Southern hybridization indicated single copy nature of the probes.
基于与鸟分枝杆菌和堪萨斯分枝杆菌缺乏杂交，从结核分枝杆菌的λgt 11文库中分离出一个重组基因组克隆。通过Southern印迹和斑点印迹杂交评估了大小为2.5 kb和2.3 kb的结核分枝杆菌DNA探针的特异性和敏感性。这些探针不会与结核分枝杆菌复合群成员以外的分枝杆菌DNA交叉杂交，也不会与非分枝杆菌来源的DNA交叉杂交。测定的敏感性为200 pg，相当于10⁴个杆菌。基因组Southern杂交表明探针具有单拷贝性质。
