镉对肾三磷酸腺苷酶的抑制作用。
Inhibition of renal adenosine triphosphatase by cadmium.
作者信息
Nechay B R, Sauners J P
出版信息
J Pharmacol Exp Ther. 1977 Mar;200(3):623-9.
The effects of CdCl2 on adenosine triphosphatase (ATPase) were studied microsomal fractions or tissue homogenates of outer cortex, inner cortex and outer medulla of dog kidney. Cd was found to be an inhibitor of Na+ +K+ Atpase with 150 value of 2.1 to 3.2 X 10(-4) M regardless of type or source of the enzyme preparation tested. Mg++ ATPase was about 10-fold less sensitive to inhibition by Cd than Na+ +K+ ATPase. The inhibition of microsomal NA+ +K+ ATPase from outer medulla was characterized by irreversible kinetics. The inhibitory effect was not altered by varying Na+ or K+ concentrations, but was decreased by disodium ethylenediaminetetracetic acid (EDTA). EDTA was more effective in preventing than in reversing the inhibition. Na+ +K+ ATPase from kidneys of several other mammalian species showed a similar sensitivity to Cd.
研究了氯化镉（CdCl₂）对犬肾外层皮质、内层皮质和外髓质微粒体部分或组织匀浆中三磷酸腺苷酶（ATPase）的影响。发现镉是Na⁺+K⁺ATPase的抑制剂，无论所测试的酶制剂类型或来源如何，其抑制常数（Ki）值为2.1至3.2×10⁻⁴M。Mg²⁺ATPase对镉抑制的敏感性比Na⁺+K⁺ATPase低约10倍。外髓质微粒体Na⁺+K⁺ATPase的抑制表现为不可逆动力学。抑制作用不会因改变Na⁺或K⁺浓度而改变，但会因乙二胺四乙酸二钠（EDTA）而降低。EDTA在预防抑制方面比逆转抑制更有效。其他几种哺乳动物肾脏中的Na⁺+K⁺ATPase对镉表现出相似的敏感性。
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