Zhou S L, Hua X X, Yang M X
Department of Parasitology, Hubei Medical College, Wuhan.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1992;10(1):5-7.
The article reports the result of the observation on the ultrastructure of the tegument and digestive tract of Schistosoma japonicum schistosomula cultured 130 days in vitro in 841 medium, and the 42-day-old adults from infected animal cultured 23 days in 851 medium by transmission electron microscopy. The results showed that the ultrastructure of the tegument including sensory papillae and flame cells was normal. The matrix seemed to have no vacuolization. The surface of the esophagus was highly rugous. Many laminae and lipid droplets filled the lumen of the digestive tract and the tubules-like depressions from basal lamina were occurred. All these structures seemed to have less variation as compared with the prior authors' data from normal worms. It is considered that the above two media which we cesigned were suitable for the growth and development of the schistosomes due to the tegument and digestive tract maintained their metabolic function in the long-term cultivation in vitro. (Figs 1-10).
本文报道了用透射电子显微镜对在841培养基中体外培养130天的日本血吸虫童虫以及从感染动物获取的42日龄成虫在851培养基中培养23天的虫体的皮层和消化道超微结构进行观察的结果。结果显示，包括感觉乳突和焰细胞在内的皮层超微结构正常。基质似乎没有空泡化。食管表面高度粗糙。许多板层和脂滴充满消化道管腔，并且出现了来自基膜的管状凹陷。与先前作者对正常虫体的数据相比，所有这些结构似乎变化较小。据认为，我们设计的上述两种培养基适合血吸虫的生长发育，因为皮层和消化道在体外长期培养中保持了它们的代谢功能。（图1 - 10）
