HARRIS T N, HARRIS S, FARBER M B
J Exp Med. 1962 Nov 1;116(5):575-88. doi: 10.1084/jem.116.5.575.
The immunologic response of neonatal and of older rabbits to the tissue (transplantation) antigens of pooled rabbit leucocytes was studied, the test system being the suppression of formation of agglutinin to Shigella paradysenteriae by transferred lymph node cells which had been incubated with Shigella antigen. In the active induction of the suppressive effect on the transferred cells it was found that neonatal rabbits reacted as vigorously as 1 kg rabbits to the prior injection of a given number of rabbit leucocytes pooled from prospective donors of lymph node cells. The suppressive effect was dose-related, and within the range of number of leucocytes used was similar for both age groups of recipients. An attempt to detect a difference in the response during the first few days after leucocyte injection, before the full suppressive effect is reached, failed to show any difference between rabbits of the two age groups. Since it had been found possible to transfer the suppressive effect passively with sera obtained from older rabbits injected with rabbit leucocytes, attempts were made to do so with sera obtained from neonatal rabbits injected with similar numbers of pooled adult rabbit leucocytes. No consistent suppression of transferred lymph node cells was observed with sera from neonatal rabbits, even with relatively large amounts of such serum. In sera of rabbits which had been injected with rabbit leucocytes at the age of 4 to 6 weeks, suppressive antibody could be detected. When anti-rabbit-leucocyte serum obtained in adult rabbits was injected into neonatal and 1 kg recipients at a given volume per gram of animal weight the suppressive effect of the serum was of similar extent in the two groups of recipients. In the adoptive transfer of the lymph node cell suppressive effect, by cells of lymph nodes draining the sites of injection of pooled rabbit leucocytes, it was found that the popliteal lymph node cells of neonatal rabbits were as effective as those of 1 kg rabbits. Splenic cells of neonatal rabbits were also effective, when an adequate number of rabbit leucocytes had been injected intravenously. Thus, in conferring adoptive immunologic response, as in active immunization, the neonatal rabbits were as effective as the older rabbits in their response to homotransplantation antigens, in contrast to the considerable difference in concentration of the suppressive antibody in sera of neonatal and older rabbits injected with rabbit leucocytes.
研究了新生兔和成年兔对混合兔白细胞组织（移植）抗原的免疫反应，测试系统是通过与志贺氏菌抗原孵育的转移淋巴结细胞抑制对副痢疾志贺氏菌凝集素的形成。在对转移细胞抑制作用的主动诱导中发现，新生兔对预先注射一定数量来自预期淋巴结细胞供体的混合兔白细胞的反应与1千克重的兔一样强烈。抑制作用与剂量相关，在所使用的白细胞数量范围内，两个年龄组的受体相似。在白细胞注射后最初几天，即在完全抑制作用达到之前，试图检测反应差异，但未显示两个年龄组的兔之间有任何差异。由于已发现用注射兔白细胞的成年兔血清可被动转移抑制作用，因此尝试用注射相似数量混合成年兔白细胞的新生兔血清进行同样操作。即使使用相对大量的新生兔血清，也未观察到对转移淋巴结细胞的一致抑制作用。在4至6周龄时注射兔白细胞的兔血清中，可检测到抑制性抗体。当以每克动物体重给定体积将成年兔获得的抗兔白细胞血清注射到新生兔和1千克重的受体中时，血清的抑制作用在两组受体中程度相似。在通过注射混合兔白细胞部位引流淋巴结的细胞进行淋巴结细胞抑制作用的过继转移中，发现新生兔的腘淋巴结细胞与1千克重兔的一样有效。当静脉注射足够数量的兔白细胞时，新生兔的脾细胞也有效。因此，在赋予过继免疫反应方面如同在主动免疫中一样，新生兔对同种移植抗原的反应与成年兔一样有效，这与注射兔白细胞的新生兔和成年兔血清中抑制性抗体浓度的显著差异形成对比。
