Kahn A, Boyer C, Cottreau D, Marie J, Boivin P
Pediatr Res. 1977 Apr;11(4):271-6. doi: 10.1203/00006450-197704000-00001.
Blood from 10 normal healthy adults and cord blood from 8 healthy full term infants were infiltrated through a mixture sulfoethylethycellulose-Sephadex G 25 in order to eliminate the platelets and the leukocytes. Then the erythrocytes were fractionated into young and old cells by centrifugation in microhematocrit tubes. The enzyme activity and the immunologic reactivity of glucose phosphate isomerase (EC.5.3.1.9), phosphoglycerate kinase (EC.2.7.2.3), pyruvate kinase (ec.2.7.1.40), glucose 6-phosphate dehydrogenase (EC. 1.1.1.49), and 6-phosphogluconate dehydrogenase (EC.1.1.1.44) were measured in every fraction. As previously reported, the enzyme activities were far higher in cord blood than in adult blood red cells; nevertheless, the age-related loss of enzyme activity was similar in both cord and adult blood. The decrease of the enzyme activity of glucose phosphate isomerase and phosphoglycerate kinase in old cells was singly associated with a lowered concentration of the enzyme-related antigen; by contrast, the age-related decrease of the enzyme activity of pyruvate kinase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase was associated with both a lowered concentration of the enzyme-related antigen and a lowered "molecular specific activity" (i.e., a lowered ratio of enzyme activity to enzyme-related antigen concentration). This phenomenon was especially marked for pyruvate kinase, which had a molecular specific activity in old cells that was 68% of that in young cells. Phosphofructokinase had a lower enzyme activity in cord blood erythrocytes than in adult blood erythrocytes; the difference was especially important in old cells from infants in which phosphofructokinase activity was 53% of that in old cells from adults. Phosphofructokinase from old cells of full term infants and from unfractionated cells from two premature infants (21 and 32 weeks of gestation) was less neutralized by anti-muscle phosphofructokinase serum and more inhibited by ATP than the enzyme from adult blood erythrocytes.
采集10名正常健康成年人的血液以及8名足月健康婴儿的脐带血，使其通过磺酸乙基纤维素-葡聚糖凝胶G 25混合物进行渗透，以去除血小板和白细胞。然后，通过在微量血细胞比容管中离心，将红细胞分为年轻细胞和衰老细胞。测定了每个组分中磷酸葡萄糖异构酶（EC.5.3.1.9）、磷酸甘油酸激酶（EC.2.7.2.3）、丙酮酸激酶（ec.2.7.1.40）、葡萄糖6-磷酸脱氢酶（EC. 1.1.1.49）和6-磷酸葡萄糖酸脱氢酶（EC.1.1.1.44）的酶活性和免疫反应性。如先前报道，脐带血中的酶活性远高于成人红细胞中的酶活性；然而，脐带血和成人血液中与年龄相关的酶活性损失相似。衰老细胞中磷酸葡萄糖异构酶和磷酸甘油酸激酶的酶活性降低仅与酶相关抗原浓度降低有关；相比之下，丙酮酸激酶、葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶与年龄相关的酶活性降低与酶相关抗原浓度降低和“分子比活性”降低（即酶活性与酶相关抗原浓度的比值降低）均有关。这种现象在丙酮酸激酶中尤为明显，衰老细胞中的分子比活性是年轻细胞中的68%。磷酸果糖激酶在脐带血红细胞中的酶活性低于成人血液红细胞中的酶活性；这种差异在婴儿衰老细胞中尤为显著，其中磷酸果糖激酶活性仅为成人衰老细胞中活性的53%。与成人血液红细胞中的酶相比，足月婴儿衰老细胞和两名早产儿（妊娠21周和32周）未分级细胞中的磷酸果糖激酶受抗肌肉磷酸果糖激酶血清的中和作用较小，且受ATP的抑制作用更大。
