Robinson B W, Venaille T, Blum R, Mendis A H
Department of Medicine, University of Western Australia, Nedlands.
Exp Lung Res. 1992 Sep-Oct;18(5):583-93. doi: 10.3109/01902149209031696.
Damage and detachment of epithelial cells is thought to contribute to the pathologenesis of asthma. Both eosinophils and neutrophils are found in asthmatic airways and several studies have suggested that eosinophils may be responsible for the epithelial cell detachment of asthma. To compare the capacity of purified human eosinophils and neutrophils to mediate epithelial cell detachment, we utilized a human amniotic epithelial cell-basement membrane model that we have recently described. Activated eosinophils induced little detachment at 4 h (less than 10% detachment), which contrasted with that seen with equivalent numbers of identically handled neutrophils (29 +/- 6% detachment, p less than .05). In contrast, eosinophils did induce damage to epithelial cells to an extent similar to neutrophils when assessed using a 51Cr release assay (17 +/- 6% and 18 +/- 9% release of 51Cr, respectively). When purified preparations of the major eosinophil-derived protein major basic protein (MBP) were studied, similar effects on epithelial cells were observed, i.e., damage (77 +/- 13% release of 51Cr) without detachment (less than 5% cell detachment). These data suggest that neutrophils are more effective in inducing detachment of human epithelial cells, whereas both eosinophils and neutrophils damage human epithelial cells.
上皮细胞的损伤和脱离被认为与哮喘的发病机制有关。在哮喘患者的气道中可发现嗜酸性粒细胞和中性粒细胞，多项研究表明嗜酸性粒细胞可能是导致哮喘患者上皮细胞脱离的原因。为了比较纯化的人嗜酸性粒细胞和中性粒细胞介导上皮细胞脱离的能力，我们采用了我们最近描述的人羊膜上皮细胞-基底膜模型。活化的嗜酸性粒细胞在4小时时诱导的脱离很少（少于10%的脱离），这与相同数量、经过相同处理的中性粒细胞所观察到的情况形成对比（29±6%的脱离，p<0.05）。相比之下，当使用51Cr释放试验进行评估时，嗜酸性粒细胞对上皮细胞造成的损伤程度与中性粒细胞相似（51Cr的释放分别为17±6%和18±9%）。当研究主要的嗜酸性粒细胞衍生蛋白——主要碱性蛋白（MBP）的纯化制剂时，观察到对上皮细胞有类似的影响，即造成损伤（51Cr释放77±13%）但无脱离（细胞脱离少于5%）。这些数据表明，中性粒细胞在诱导人上皮细胞脱离方面更有效，而嗜酸性粒细胞和中性粒细胞都会损伤人上皮细胞。
