Yaremchuk A D, Tukalo M A, Krikliviy I, Malchenko N, Biou V, Berthet-Colominas C, Cusack S
Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukraine, Kiev.
FEBS Lett. 1992 Sep 28;310(2):157-61. doi: 10.1016/0014-5793(92)81319-h.
Two distinct complexes between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 A, b = 126.8 A, c = 197.1 A, beta = 132.4 degrees and diffract to about 3.5 A. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 124.5 A, b = 128.9 A, c = 121.2 A and diffract to 2.8 A resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer.
利用硫酸铵作为沉淀剂，已使嗜热栖热菌的丝氨酰 - tRNA合成酶与tRNA(Ser)之间形成的两种不同复合物结晶。III型晶体从含有合成酶二聚体与tRNA化学计量比为1:2.5的溶液中生长。它们属于单斜空间群C2，晶胞尺寸为a = 211.6 Å，b = 126.8 Å，c = 197.1 Å，β = 132.4°，衍射极限约为3.5 Å。初步晶体学结果表明，晶体学不对称单元包含两个合成酶二聚体。IV型晶体从含有合成酶二聚体与tRNA化学计量比为1:1.5的溶液中生长。它们属于正交空间群P2(1)2(1)2(1)，晶胞尺寸为a = 124.5 Å，b = 128.9 Å，c = 121.2 Å，衍射分辨率为2.8 Å。初步晶体学结果表明，这些晶体仅包含一个与合成酶二聚体结合的tRNA分子。
