Yamada H, Mizuguchi J, Noji M, Nakanishi M
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
FEBS Lett. 1992 Sep 28;310(2):201-3. doi: 10.1016/0014-5793(92)81329-k.
Using a digital imaging fluorescence microscope we have observed the membrane immunoglobulin (mIg)-induced desensitization of calcium signals in individual BAL17 B lymphoma cells which express two kinds of antigen receptors, mIgM and mIgD. The mIgD-mediated desensitization was partly abrogated by pretreating the cells with phorbol 12-myristate 13-acetate (PMA) for 24 h, however, the mIgM-mediated one was not affected by the pretreatment. This supports the idea that at least two mechanisms are operative for mIg-induced desensitization in B cells.
使用数字成像荧光显微镜，我们观察到在表达两种抗原受体mIgM和mIgD的单个BAL17 B淋巴瘤细胞中，膜免疫球蛋白（mIg）诱导的钙信号脱敏。用佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）预处理细胞24小时，可部分消除mIgD介导的脱敏作用，然而，mIgM介导的脱敏作用不受该预处理的影响。这支持了至少有两种机制在B细胞中mIg诱导的脱敏过程中起作用的观点。
