Scotter M J, Thorpe S A, Reynolds S L, Wilson L A, Lewis D J
Food Science Laboratory, Ministry of Agriculture, Fisheries and Food, Norwich, UK.
Food Addit Contam. 1992 May-Jun;9(3):237-42. doi: 10.1080/02652039209374067.
Retail samples of livers from calf (23), ox (18), lamb (17), pig (15), chicken (16) and turkey (1) were analysed to determine levels of vitamin A (all trans-retinol) and to aid assessment of the effects of using vitamin supplemented compound feedingstuffs for livestock. For comparison, 22 liver samples from lambs reared on diets not containing vitamin-supplemented compound feedingstuffs and four samples of liver from ox which had received supplemented feed but not during the last four months prior to slaughter were also analysed. The chosen method of analysis utilized saponification, solvent extraction and normal-phase high performance liquid chromatography with fluorescence detection. For all species analysed, the levels of vitamin A ranged from 10 to 1100 mg/kg, with all but seven at or below 400 mg/kg. For lamb and ox livers, the mean levels were 310 mg/kg and 200 mg/kg respectively for retail samples. The mean levels were 220 mg/kg (lamb) and 120 mg/kg (ox) in liver samples from animals fed controlled diets. The results are of the same order as those reported over recent years.
对来自小牛(23份)、牛(18份)、羔羊(17份)、猪(15份)、鸡(16份)和火鸡(1份)的肝脏零售样本进行了分析,以确定维生素A(全反式视黄醇)的含量,并协助评估使用补充维生素的复合饲料对牲畜的影响。作为比较,还分析了22份来自未食用补充维生素复合饲料的羔羊肝脏样本,以及4份来自牛的肝脏样本,这些牛在屠宰前的最后四个月内未食用补充饲料,但此前曾食用过。所选用的分析方法包括皂化、溶剂萃取和带荧光检测的正相高效液相色谱法。对于所有分析的物种,维生素A的含量范围为10至1100毫克/千克,除7份样本外,其余均在400毫克/千克及以下。对于羔羊和牛肝脏的零售样本,平均含量分别为310毫克/千克和200毫克/千克。在食用对照饲料的动物肝脏样本中,平均含量为220毫克/千克(羔羊)和120毫克/千克(牛)。结果与近年来报道的结果处于同一水平。