丙酸和丁酸对大鼠肝细胞中乙酸代谢的影响。

The effects of propionate and butyrate on acetate metabolism in rat hepatocytes.

Gordon M J, Crabtree B

Rowett Research Institute, Aberdeen, Scotland, U.K.

Int J Biochem. 1992 Jul;24(7):1029-31. doi: 10.1016/0020-711x(92)90369-c.

Abstract

Two mM propionate or butyrate inhibited the mitochondrial uptake of acetate by rat hepatocytes. 2. With propionate the inhibition was so strong that the net formation of acetate in the cytoplasm, usually masked by the mitochondrial uptake, appeared directly as a net output of acetate into the medium; showing that this net formation of acetate, reported by [Crabtree B., Gordon M.-J. and Christie S. L. (1990) Biochem. J. 270, 219-225] is not an artefact arising from a misinterpretation of isotopic data. 3. The results also suggest that propionate and butyrate inhibit peroxisomal metabolism.

摘要

2毫摩尔的丙酸盐或丁酸盐可抑制大鼠肝细胞对乙酸盐的线粒体摄取。2. 对于丙酸盐，这种抑制作用非常强烈，以至于细胞质中乙酸盐的净生成（通常被线粒体摄取所掩盖）直接表现为乙酸盐向培养基中的净输出；这表明[克拉布特里B、戈登M-J和克里斯蒂S-L（1990年）《生物化学杂志》270卷，219 - 225页]所报道的乙酸盐的这种净生成并非源于对同位素数据的错误解读而产生的假象。3. 结果还表明丙酸盐和丁酸盐可抑制过氧化物酶体代谢。