滴虫原生动物的核糖体具有原核生物特征。

Ribosomes from trichomonad protozoa have prokaryotic characteristics.

Champney W S, Chittum H S, Samuels R

Department of Biochemistry, East Tennessee State University, Johnson City 37614.

Int J Biochem. 1992 Jul;24(7):1125-33. doi: 10.1016/0020-711x(92)90383-c.

Abstract

Ribosomes from cells of the genera Trichomonas and Tritrichomonas have been isolated and characterized. The ribosomes from each organism had a sedimentation coefficient of 70S in calibrated sucrose gradients and the subunits sedimented as 50S and 30S particles under the same conditions. 2. The major ribosomal RNAs from each species were identical in size to prokaryotic ribosomal RNAs when examined by denaturing gel electrophoresis. The ribosomes contained both 5.8S and 5S RNAs. 3. The ribosomal proteins were compared by the methods of two-dimensional gel electrophoresis and reversed phase HPLC. Electrophoresis of the ribosomal proteins in two different gel systems indicated the presence of 56 proteins in T. gallinae, 40 in T. bactrachorum and 45 in the Tritrichomonas sp. The protein molecular mass range was 8.5-40 kDa. 4. The HPLC analysis confirmed the protein number established by the gel methods. 5. Both methods of analysis revealed greater similarities between the ribosomal proteins of the 2 Tritrichomonas sp. than between those of the more distantly related T. gallinae and T. bactrachorum.

摘要

已分离并鉴定了来自毛滴虫属和三毛滴虫属细胞的核糖体。在校准的蔗糖梯度中，每个生物体的核糖体沉降系数为70S，在相同条件下，亚基沉降为50S和30S颗粒。2. 通过变性凝胶电泳检测，每个物种的主要核糖体RNA大小与原核核糖体RNA相同。核糖体含有5.8S和5S RNA。3. 通过二维凝胶电泳和反相高效液相色谱法对核糖体蛋白进行了比较。在两种不同的凝胶系统中对核糖体蛋白进行电泳表明，鸡三毛滴虫中有56种蛋白质，巴氏三毛滴虫中有40种，三毛滴虫属中有45种。蛋白质分子量范围为8.5 - 40 kDa。4. 高效液相色谱分析证实了凝胶法确定的蛋白数量。5. 两种分析方法均显示，两个三毛滴虫属的核糖体蛋白之间的相似性大于亲缘关系较远的鸡三毛滴虫和巴氏三毛滴虫之间的相似性。