从加热的60S核糖体亚基释放的5S - RNA - 蛋白质复合物的表征及性质

Characterization and properties of a 5S-RNA-protein complex released from heated 60S ribosomal subunits.

作者信息

Reboud A M, Dubost S, Reboud J P

出版信息

Eur J Biochem. 1984 Sep 3;143(2):303-7. doi: 10.1111/j.1432-1033.1984.tb08372.x.

DOI:10.1111/j.1432-1033.1984.tb08372.x

Abstract

When rat liver 60S ribosomal subunits were heated in phosphate buffer in the presence of MgCl2, 5S RNA was released in the form of a nucleoprotein complex (RNPH), which was isolated either by electrophoresis in polyacrylamide gel or centrifugation through a sucrose gradient. In addition to L5 several proteins of functional significance were identified in the complex: the acidic phosphoproteins P1-P2 and, as weaker spots, L3-L4, L6-L7 and L22. Most of these proteins were also found, but only as traces, in the RNPEDTA used as a control. RNPH was able to associate with 40S subunits. Our results support the interpretation that RNPH is located at the subunits' interface, at or near the peptidyl-transferase center.

摘要

当大鼠肝脏60S核糖体亚基在MgCl2存在的情况下于磷酸盐缓冲液中加热时，5S RNA以核蛋白复合物（RNPH）的形式释放出来，该复合物可通过聚丙烯酰胺凝胶电泳或蔗糖梯度离心进行分离。除了L5之外，在复合物中还鉴定出了几种具有功能意义的蛋白质：酸性磷蛋白P1 - P2，以及较弱条带的L3 - L4、L6 - L7和L22。作为对照的RNPEDTA中也发现了这些蛋白质中的大多数，但只是痕量存在。RNPH能够与40S亚基结合。我们的结果支持这样一种解释，即RNPH位于亚基界面，在肽基转移酶中心或其附近。