维生素K3在豚鼠和大鼠心脏组织中的单电子和双电子还原反应。
One- and two-electron reduction of menadione in guinea-pig and rat cardiac tissue.
作者信息
Floreani M, Carpenedo F
机构信息
Department of Pharmacology, University of Padova, Italy.
出版信息
Gen Pharmacol. 1992 Jul;23(4):757-62. doi: 10.1016/0306-3623(92)90162-d.
PMID:1397983
Abstract
- In both guinea-pig and rat heart, mitochondrial NADH-ubiquinone-reductase and soluble DT-diaphorase accounted for 49-50% and 48-50% of menadione metabolism, respectively. Microsomal NADPH-cytochrome P450-reductase was responsible for less than 1% of menadione reduction. 2. Menadione was a high-affinity substrate for all reductases (Km values from 1 to 10 microM). 3. Marked amounts of O2-. (superoxide anion) were generated as a consequence of cardiac metabolism of menadione. 4. Menadione-induced O2-. generation was about 3-fold higher in guinea-pig than in rat heart. 5. All results were compared with data obtained on guinea-pig and rat liver.
摘要
- 在豚鼠和大鼠心脏中，线粒体NADH-泛醌还原酶和可溶性DT-黄递酶分别占甲萘醌代谢的49 - 50%和48 - 50%。微粒体NADPH-细胞色素P450还原酶对甲萘醌还原的贡献率不到1%。2. 甲萘醌是所有还原酶的高亲和力底物（Km值为1至10微摩尔）。3. 甲萘醌在心脏代谢过程中会产生大量的O2-（超氧阴离子）。4. 甲萘醌诱导产生的O2-在豚鼠心脏中比在大鼠心脏中高约3倍。5. 所有结果均与在豚鼠和大鼠肝脏上获得的数据进行了比较。
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