Atkin A L, Henry R W, Roy K L, Bell J B
Department of Genetics, University of Alberta, Edmonton, Canada.
Gene. 1992 Sep 21;119(1):57-63. doi: 10.1016/0378-1119(92)90066-x.
The purpose of this work was to examine the tRNA(Trp)-encoding genes (tRNA(Trp)) of Saccharomyces cerevisiae to gain insight as to why tRNA(Trp) amber suppressors, isolated by conventional genetic techniques, have not been reported. The results herein indicate that the haploid yeast genome contains six tRNA(Trp) genes which map to five or six chromosomes. Not only do the six genes have identical coding sequences but their introns are also identical. Gene replacement experiments indicate that five copies of tRNA(Trp) are sufficient for cell viability. Thus, mutation of one tRNA(Trp) gene to a suppressor in vivo, lowering the functional number of tRNA(Trp) genes, would not be expected to be lethal.
这项工作的目的是研究酿酒酵母中编码色氨酸的转运RNA基因（tRNA(Trp)），以深入了解为何通过传统遗传技术分离出的tRNA(Trp)琥珀抑制子尚未见报道。本文的结果表明，单倍体酵母基因组包含六个tRNA(Trp)基因，这些基因定位在五或六条染色体上。这六个基因不仅具有相同的编码序列，而且它们的内含子也相同。基因置换实验表明，五个tRNA(Trp)拷贝足以维持细胞活力。因此，体内一个tRNA(Trp)基因突变为抑制子，降低了tRNA(Trp)基因的功能数量，预计不会导致致死。
