Atkin A L, Roy K L, Bell J B
Department of Genetics, University of Alberta, Edmonton, Canada.
Mol Cell Biol. 1990 Aug;10(8):4379-83. doi: 10.1128/mcb.10.8.4379-4383.1990.
In vitro mutagenesis was used to create putative opal suppressor alleles of a tRNA(Trp) gene of Saccharomyces cerevisiae. The construct with the requisite anticodon change did not result in an active suppressor, whereas when a second change was introduced into the portion of the gene encoding the intron, an active and specific opal suppressor was produced. We propose that the secondary structure of transcripts from the first mutant may prevent efficient pre-tRNA processing, whereas normal processing occurs with the double mutant.
利用体外诱变技术构建了酿酒酵母tRNA(Trp)基因的假定乳白抑制子等位基因。带有必要反密码子变化的构建体并未产生活性抑制子，而当在编码内含子的基因部分引入第二个变化时，则产生了活性且特异的乳白抑制子。我们推测，第一个突变体转录本的二级结构可能会阻止有效的前体tRNA加工，而双突变体则能进行正常加工。
