del Castillo Agudo L, Nieto Soria A, Sentandreu R
Departamento de Microbiología, Universidad de Valencia, Spain.
Gene. 1992 Oct 12;120(1):59-65. doi: 10.1016/0378-1119(92)90009-e.
Invertase (INV) is encoded in Saccharomyces cerevisiae by a family of genes, comprising SUC1-SUC5 and SUC7. Production of INV is highly variable, dependent on the strain and SUC gene present in the cell. The differences in INV production derive from the structure of the genes or are dependent on the genetic background of the strain. Centromeric plasmids (based on YCp50) carrying one of the SUC genes (except SUC7) were introduced into a strain (SEY2101) lacking SUC genes. The INV produced by the transformants was dependent on the individual SUC genes, and correlated with INV mRNA levels. Plasmids in which SUC2 had been placed under control of promoters from the other SUC genes, were used to transform SEY2101 cells. The amounts of INV produced by cells carrying hybrid SUC genes were in agreement with the levels expected if the promoter controlled the expression of the SUC2 structural region. It is suggested that the differences in expression are a function of the transcription efficiency of the different SUC gene promoters, based on the divergence of 5' sequences.
在酿酒酵母中，转化酶（INV）由一个基因家族编码，包括SUC1 - SUC5和SUC7。INV的产量高度可变，取决于细胞中存在的菌株和SUC基因。INV产量的差异源于基因结构或取决于菌株的遗传背景。携带其中一个SUC基因（SUC7除外）的着丝粒质粒（基于YCp50）被导入缺乏SUC基因的菌株（SEY2101）。转化体产生的INV取决于单个SUC基因，并与INV mRNA水平相关。将SUC2置于其他SUC基因启动子控制下的质粒用于转化SEY2101细胞。携带杂交SUC基因的细胞产生的INV量与如果启动子控制SUC2结构区域的表达所预期的水平一致。基于5'序列的差异，表明表达差异是不同SUC基因启动子转录效率的函数。
