Liu F, Bateman E
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405-0068.
Gene. 1992 Oct 21;120(2):143-9. doi: 10.1016/0378-1119(92)90087-6.
We have developed and characterized an efficient in vitro system from the protozoan, Acanthamoeba castellanii, that accurately initiates transcription from the adenovirus-2 major late promoter (AdMLP). Transcription by A. castellanii RNA polymerase II (pol II) is initiated at the same nucleotide (nt) that is used by HeLa extracts and is dependent upon adenovirus sequences located between nt -51 and the region around the transcription start point (tsp). The results suggest that the A. castellanii transcription factors for pol II which determine the tsp and the promoter elements that they recognize have been functionally conserved during evolution.
我们已经从原生动物卡氏棘阿米巴开发并鉴定了一种高效的体外系统，该系统能准确地从腺病毒2型主要晚期启动子（AdMLP）起始转录。卡氏棘阿米巴RNA聚合酶II（pol II）的转录起始于与HeLa提取物相同的核苷酸（nt），并且依赖于位于nt -51与转录起始点（tsp）周围区域之间的腺病毒序列。结果表明，在进化过程中，卡氏棘阿米巴中决定tsp的pol II转录因子及其识别的启动子元件在功能上是保守的。
